ABSTRACT
The particles are so highly reactive that any kind of targeting or achieving larger volume of biodistribution is practically impossible. Polymerization attaches on each surface group 2 more monomers, and polyamidoamide spherical polymers grow in shells. Combination complexes are structures in which DNA is condensed with conjugates having both a ligand for cell attachment/endocytosis and/or an endosomolytic agent such as synthetic peptide, fusogenic protein, or adenovirus. Transferrin and insulin ligands can target corresponding receptors in a wide range of cells. Chemistry developed in the targeting of liposomes was successfully applied to targeting of lipid-DNA complexes. Asialofetuin is a glycoprotein having triantennary galactose-terminated sugar chains and is an excellent ligand for selective recognition of asilaoglycoprotein receptor of rodent and human hepatocytes. Most researchers are trying to assemble an artificial virus in which condensed DNA would be encapsulated, possibly with the addition of endosome-disrupting agents.
