ABSTRACT
In vitro percutaneous absorption methods are used for various reasons. Often it
may be the only ethical way of obtaining human skin absorption data with
potentially toxic chemicals. These studies also facilitate simultaneous measure-
ment of skin metabolism which can be examined without metabolic interference
from systemic organs (See Chapter 33, “Methods for in vitro skin metabolism
studies”). The use of animals is also minimized with in vitro studies since many
diffusion cells can be assembled from the skin of one animal.
