Analysis of Gene Expression at the Functional Genomic Level

Introduction ..........................................................................................................247 Principles and General Considerations ................................................................248 Isolation of Total RNAs and Purification of mRNAs ..........................................250 Electrophoresis of RNAs Using Formaldehyde Agarose Gels ............................250 Blotting of RNAs onto Nylon Membranes by the Capillary Method ................253 Preparation of Isotopic or Nonisotopic DNA/RNA Probes ................................253