ABSTRACT

Introduction ..........................................................................................................461 Isolation of Total RNA from Cells or Tissues .....................................................462 Preparation of Fluorescent Probes from mRNA ..................................................463 Generation of DNA Microarrays or Chips ..........................................................465 Prehybridization of Gene Chips or Arrays ..........................................................465 Hybridization ........................................................................................................466 Washing ................................................................................................................466 Scanning Gene Chips Using GenePix 4000a Microarray Scanner .....................467 References ............................................................................................................468