ABSTRACT
Abstract ....................................................................................................................29 3.1 Introduction ....................................................................................................30 3.2 Overview of Approach...................................................................................30 3.3 Bioinformatics Analysis of APP....................................................................31 3.4 Biological Roles of GFD ...............................................................................32 3.5 Previous Crystallization Studies ....................................................................32 3.6 Expression of Recombinant GFD in
Pichia pastoris
....................................32 3.6.1 Materials.............................................................................................33 3.6.2 Method for Cloning ...........................................................................33 3.6.3 Method for Expression.......................................................................34
3.7 Purification of GFD .......................................................................................34 3.7.1 Method ...............................................................................................34
3.8 Crystallization of GFD...................................................................................35 3.8.1 Materials.............................................................................................36 3.8.2 Method ...............................................................................................36
3.9 Discussion ......................................................................................................37 Acknowledgments....................................................................................................39 References................................................................................................................39
