ABSTRACT
Often, the sets of kinetic parameter values available in the literature on a given enzyme are diverse, having been obtained with many different experimental techniques and under a range of experimental conditions. It is sometimes difficult to reach a satisfactory conclusion regarding which values in a set of kinetic parameters to use in a computer model that involves the particular enzyme. In addition, the sets of parameters are usually incomplete and are insufficient to define unambiguously the underlying reaction mechanism. Therefore, a systematic approach is needed to arrive at a set of parameter values, preferably unitary rate constants, that are consistent with the proposed mechanism of the enzyme and fit in with the known values of the steady-state parameters.
