ABSTRACT
One of the most powerful applications of FT-IR spectroscopy is computer-based difference spectroscopy. Difference spectroscopy is a computational procedure to calculate the spectrum of a pure component from the spectra taken from different compositions, using a primitive mathematical technique, substraction of two spectra. A polymer which is capable of bonding with organic acids could be used as adsorbing medium to improve the efficiency and specificity of acid recovery from solvents. Determinations of fresh milk components that is fat, protein, and sugar, by classical methods are already established. Different methods are applied for each component and each method requires its own unique pretreatment of the milk sample. The derivative type line in the difference spectrum appears when the sample line position is shifted from the reference position, the shift being due most probably to the molecular interaction among the components in the mixture.
