ABSTRACT
The enzyme has been called penicillium B, notatin, and glucose aerodehydrogenase, but the trivial name suggested by the Commission on Enzyme Nomenclature is glucose oxidase. Glucose oxidase has rather high specificity for β-d-glucopyranose. There is an absolute requirement for a hydroxyl group at C (1) and the activity is about 160 times higher if the hydroxyl group is in the β-position. By 1948 it was known that glucose oxidase does not oxidize β-d-glucose by direct combination of molecular oxygen with the β-d-glucose. Glucose oxidase in the oxidized form has an absorbance maximum at 280 nm, typical of all proteins, and absorbance maxima at 377 and 455 nm due to flavin adenine dinucleotide. Activity of glucose oxidase can be determined by incorporation of peroxidase and a chromogen, such as o-dianisidine, into the reaction mixture.
