ABSTRACT
The underlying principle is the viscosity change in the cytoplasm. Since spindle formation is dependent on the viscosity balance between cytoplasmic and spindle constituents, a change in cytoplasmic viscosity brings about a destruction of the spindle mechanism with the chromosomes remaining not attached to any binding force within the cell. In general, the drug is exceptionally suitable for the study of chromosome structure and metaphase arrest, under strict control over the concentration and period of treatment in almost all plant tissues. The structural integrity of the chromosome must be maintained intact. Precipitation of the chromatin matter is essential to render the chromosome visible and to increase its basophilia in staining. Crystal violet is widely used as chromosome stain. The most suitable differentiating agent for crystal violet is clove oil. It removes superfluous stain from the cytoplasm, thus rendering the stain in the chromosomes brighter, and also completing the dehydration.
