![Separation and Determination of Polynuclear Aromatic Hydrocarbons and Benzo[a]pyrene: Separation and Microanalysis of Airborne Particulate Matter for Benzo[a]pyrene Using Thin Layer Chromatography and Spectrofluorimetry](https://images.tandf.co.uk/common/jackets/crclarge/978036758/9780367580230.jpg "Separation and Determination of Polynuclear Aromatic Hydrocarbons and Benzo[a]pyrene: Separation and Microanalysis of Airborne Particulate Matter for Benzo[a]pyrene Using Thin Layer Chromatography and Spectrofluorimetry")
ABSTRACT
The particulate matter collected from the urban atmosphere is extracted with an organic solvent and then separated alongside pure benzo[a]pyrene (BaP) with alumina thin-layer chromatography. The unknown and standard spots are eluted, their solutions are evaporated, and the residues are dissolved in concentrated sulfuric acid. An intensely yellow fluorescent cationic salt is obtained. The concentration of benzo[a]pyrene is measured using the fluorescence method described in Method 102C.
