ABSTRACT
This chapter is a natural extension of our earlier studies of restriction enzymes, which are essential tools in cloning. Cloning is a means of producing recombinant DNA molecules, new molecules which are formed from already existing ones. A collection of clones containing inserts that are DNA fragments from the genome of an organism is called a clone library. The vectors can only accept inserts within a certain size range dependent on the vector. This fact limits the DNA that can be put into a vector and, hence, the DNA that can be contained in a library. The cloning vector is cut with a restriction enzyme, and a piece of DNA is inserted into the cut. Then the vector is transferred into the host where it can be replicated into an experimentally useful quantity of DNA. A collection of clones containing inserts that are DNA fragments from the genome of an organism is called a clone library.
