ABSTRACT
This chapter considers the most straightforward method of physical mapping, overlapping random clones. To begin, a genomic library is constructed. The chapter assumes here the library that represents the genome and that all portions of the genome are equally likely to be cloned. The idea in mapping by random clone overlap is that clones are chosen at random and characterized in one of several ways. The characteristics are summarized in what biologists call a fingerprint. Many fingerprinting schemes involve digesting the cloned DNA with restriction enzymes. The practical aspects of random clone mapping depends on the biology of the organism being mapped as well as the cloning vector. The fingerprint used by Kohara et al. is referred to below as a type c fingerprint-each clone was mapped for each of eight enzymes. The clones were overlapped by comparing the clone maps.
