ABSTRACT
Chromatography is used essentially for separation of compounds in various mixtures. The application of Chromatography for physicochemical measurements, however, is not new. For example two excellent books [1,2] discuss the applications of gas Chromatography (GC) for such measurements. The applications of high-performance liquid Chromatography (HPLC) for physicochemical measurements, however, is not so widespread. The quantitative relation between the chemical structure, physicochemical properties, and chromatographic retention have been discussed in detail [3]. The quantitative correlations can be used for the estimation of several physicochemical properties from the measured retention data of compounds. The application of HPLC for the determination of molecular interactions has also been reviewed [4].
