ABSTRACT
The major sources of poor reproducibility of results (i.e., RF values, development time, zone shape, detection characteristics, etc.) in TLC can be broadly characterized as contamination, poor technique, and variations in chromatographic materials and/or conditions. Geiss (1987), Smith et al. (1973), and Jork et al. (1990) listed the following parameters that can influence a TLC separation. These parameters should be recorded as completely as possible as part of the documentation procedure for TLC results (Chapter 9).
