ABSTRACT
Preparative layer chromatography (PLC) is used to separate and isolate larger amounts of material than are normally separated by analytical thin-layer chromatography. The amounts handled are usually 10-500 mg or occasionally 1 g or more. The material is isolated for the purpose of further chromatography; analytical studies such as melting point determination or infrared (IR), nuclear magnetic resonance (NMR), ultraviolet (UV) or mass spectrometry for identification; use as purified material for biological activity or chemical studies (e.g., synthesis); or obtaining pure standards for comparison with unknown samples.
