ABSTRACT
The basic experimental equipment for Field-Flow Fractionation (FFF) is, except for the channel, identical with the equipment for liquid chromatography. It is usually composed of a solvent reservoir, a pump, and an injection system; a chromatographic column is replaced by the FFF channel, followed by a detector and a registration device. The FFF channel can require additional devices, such as a centrifuge for sedimentation FFF or a power supply and other electronic regulation devices for electrical FFF. A modified device utilizes a short thermal pulse. The local heating of the solvent at a given point of the capillary is registered by a sensitive thermocouple placed in a determined distance along the capillary. Photometric detectors constitute a very large group based on light interaction with the mass of detected macromolecular or particulate solute. This interaction gives rise to absorption of light, fluorescence, optical rotation, or light scattering.
