ABSTRACT
Liquid chromatography separations fall broadly into two classes: those where retention is energetically controlled and those where retention is entropically controlled. Entropically driven separations will include both size exclusion chromatography and chiral chromatography. All the solutes in exclusion chromatography, unless significantly retained by interactive forces, are eluted between the interstitial column volume and the column dead volume. The stationary phase consisted of semi-rigid spherical beads about 20–40 µm in diameter having a mean pore diameter of 50 Å. The material was formed from the co-polymerization of ethylene glycol and methacrylates. The separation has been carried out on a silica bonded phase carrying (R)-dinitrobenzoyl phenylglycine. Dispersive stationary phases are those commonly called reversed phases and contain hydrocarbon chains the length of which is chosen to suit the particular sample being separated. As a result of the strong dispersive interactions possible with long hydrocarbon chains, proteins can be denatured during the chromatographic development.
