ABSTRACT
Polymeric compounds of vitamins attract attention both as donors of these substances, indispensable for the normal functioning of a cell, and as affinity chromatography sorbents. Processes involved in the ionic interaction of vitamins and carriers play an important role in the refining of these naturally occurring substances. Some studies deal with synthesizing polymeric compounds of a widespread vitamin of the pteroylglutamic (folic) acid, used as affinity carriers to isolate various enzymes: dihydrofolate reductase, phenylalanine hydroxylase and other proteins. Affinity sorbents for hydroxymethyl transferase and dihydrofolate reductase with the bound folic acid have been described in a number of papers. Study deals with obtaining immobilized folic acid using epoxy-containing Sepharose. Polyglutamates of the folic acid have been synthesized using chloroformated polystyrene as a solid phase.
