ABSTRACT
A detailed study of the behaviour of macromolecules near the critical conditions in a wide range of molecular weights and pore sizes has made it possible to establish a number of universal relationships between the change in Kd and the variation of the size of molecules and pores and of the solvent composition. Disregarding the spatial interactions of segments is justified for short chains. The hatched areas on the chromatograms correspond to the calculated retention volumes in the zones of differing functionalities, with the real molecular weights in each zone accounted. Though such an approach allowed explaining and describing quantitatively from a unified viewpoint the main experimental data available in literature on determining the FTD of linear reactive oligomers both in isocratic and in gradient separation modes, the use of tabular values of correlation parameters as applied to a segment should be considered rather as an illustration of the potentialities of the proposed method in calculating the retention volumes.
