ABSTRACT
This chapter is about common calculations associated with animal cell culture. In animal cell culture, living cells derived from the tissue of multicellular animals are maintained in cell culture medium inside petri-style dishes, the wells of multi-well plates, flasks, or other vessels, Figures 21.1–21.3. Maintaining cells outside of an organism requires stringent conditions of bacterial sterility, appropriate nutrition, constant temperature, and the like. There 392are a vast number of applications of cell culture, ranging from basic research into the intricacies of cellular function to the production of large quantities of commercially valuable drugs and enzymes.
