ABSTRACT
As the number of enzyme species increases, for example, in complex kinetic mechanisms, then the number of King-Altman interconversion patterns increases accordingly. Attempts have been made to reduce the tedium of the method in complex situations, and several variations of the basic King-Altman procedure have been suggested which lead to some simplification in the use of the method. This chapter discusses those suggested by Volkenstein and Goldstein. The easiest modification of the King-Altman method is restricted to cases having multiple lines connecting two points in one or both directions. The lines for a given direction may simply be added. Another simplification of complex patterns in the King-Altman method involves reduction of portions of a complex pattern to a point represented by the appropriate matrix solution. The main objects of any statistical analysis of enzyme kinetics data are to obtain accurate estimates of the kinetic parameters of the selected mathematical model and obtain a measure of the precision of the results.
