ABSTRACT
Only in the simplest cases is it possible to determine the values of the individual rate constants from a study of the kinetics of an enzyme reaction with the use of initial rate measurements. Difficulties will arise, however, if the reaction proceeds very rapidly, since the time required for mixing the reactants may be comparable to the half-life of the reaction, and the speed at which mixing may be accomplished sets an upper limit to the rate of the reaction steps that may be isolated and measured in this manner — by the static method — which is usually much too slow for the direct measurement of most enzymatic individual reaction steps. As an application of the continuous-flow technique, the chapter discusses a particular design of the apparatus which was utilized in a kinetic study of the oxidation by molecular oxygen of the cytochrome chain of intact yeast cells, by G. D. Ludwig et al.
