ABSTRACT
This chapter covers highly-parallel studies of both RNA transcripts and of proteins. In addition to sequencing the human genome, the HGP had three other goals: to develop mapping and sequencing technologies; to carry out genome projects for five model organisms; and to investigate the ethical, legal, and societal implications. Microarray-based expression analyses are often organized so as to compare two or more highly-related cellular or tissue sources that differ in an informative way. Analyses of single cells from dissociated tissue or even in situ analysis should provide a clearer picture, giving a full description of the expression profiles of individual cells, the dynamic states within each cell type, and the proportion of, and spatial relationships of, the various cell types. Cancers are defined by natural selection acting at the level of the cell to promote abnormal proliferation. Different methods have been used to isolate single cells.
