ABSTRACT
Gel filtration chromatography works on a principle that is connected to the topic of polyacrylamide gels, and also agarose gels. In gel filtration, there are beads of resin, and each bead has a number of cracks, pores, and crevasses. The resulting product will be long fibers of polyacrylamide stretching across or along the container, and it is called a kind of “gel”. Simple polyacrylamide fibers do not hold together well, and large molecules with a lot of pressure behind them will easily push the fibers apart. A growing polyacrylamide chain that adds a molecule of bis-acrylamide will become crosslinked to another polyacrylamide chain. Unlike column chromatography, we cannot achieve separation of macromolecules simply by flowing a buffer the polyacrylamide gel. A gel that was 15% acrylamide might do a very good job of slowing these smaller proteins down, but it would be so dense that larger proteins would not be able to penetrate at all.
