ABSTRACT
The different kinds of assays that exist have an extraordinary range of types. There are assays by color, assays by the activity of an enzyme, activity by binding to some ligand, assays by immunochemistry, and far more. Before ever attempting a purification of a protein, work out how we are going to find it. The name comes from the glassware used to develop the first assays for enzymes: only cocktail glasses were available, so they were used. Phosphofructokinase-1 is an enzyme that is part of the glycolysis pathway. Other reporter systems include chromogenic enzymes such as alkaline phosphatase and horseradish peroxidases, or even radiolabelled antibodies detected by radioimaging. Some enzymes can be assayed without any spectroscopic equipment at all. Determine the total activity for each enzyme from each source. Catalase is one such enzyme. Catalase converts hydrogen peroxide into water and oxygen.
