ABSTRACT
II. Production of Anionic Rhamnolipids with Free Cells of Pseudomonas sp. DSM 2874 101
III. Production of Rhamnolipids with Immobilized Cells 102 A. Development of the biocatalytic system and screening for
an optimal support system 103 B. Optimization of reaction parameters 106 C. Development of an immobilization procedure suitable for the
large-scale entrapment of living cells into a Ca-alginate support 114
IV. Development of a Process Mode for the Continuous Production of Rhamnolipids 115 A. Isolation of rhamnolipids based on flotation 115 B. Isolation of the rhamnolipids by adsorption onto an amberlite
XAD-2 support 116 C. Construction of a fluidized bed reactor 117 D. Process mode for production and continuous isolation 118 E. Production in a fluidized bed reactor 123 F. Regeneration of the immobilized biocatalysts 127 G. Further development of the fluidized bed reactor 128
References 131
I. INTRODUCTION Industrial processes with microorganisms are generally based on the exploitation of free, growing, or resting cells. However, in the last years, many publications seemed to indicate that immobilized cell systems are gaining importance in the development of biotechnological processes [1].
