ABSTRACT
Chromatographic Methods.......................................................................... 83 4.3 Selection of Application Conditions ....................................................................... 84
4.3.1 Solvent Considerations................................................................................ 84 4.3.2 Factors Related to the Column and Sample ............................................... 85 4.3.3 Description of Sample Retention................................................................ 86
4.4 Selection of Elution Conditions.............................................................................. 88 4.4.1 General Approaches for Elution ................................................................. 88 4.4.2 Selection of Elution Buffers ....................................................................... 88 4.4.3 Description of Sample Elution.................................................................... 90
4.5 Column Regeneration.............................................................................................. 93 4.5.1 Choice of Regeneration Conditions............................................................ 93 4.5.2 Description of Column Regeneration ......................................................... 93
4.6 Summary and Conclusions ..................................................................................... 94 Symbols and Abbreviations .............................................................................................. 94 Acknowledgments............................................................................................................. 95 References ......................................................................................................................... 95
4.1 INTRODUCTION
The previous chapters have examined the support materials and immobilization methods that are often used in affinity chromatography, and the ligands used in these columns will be discussed in Chapters 5 to 10. However, another set of factors in the use of affinity chromatography must also be considered: the application and elution conditions. If these conditions are not selected properly, the target of interest may not bind to the affinity column or may not be recovered in an appropriate form.
