ABSTRACT

Metabolic profiling of leaf extract of Ocimum species revealed the identification of fifty phytochemicals including twenty eight flavonoids, four propenyl phenol subsidiaries, two triterpenic acids, eleven phenolic acids and five phenolic acid esters. Twenty three phytochemicals were unambiguously identified and characterized by comparing their retention time and fragmentation pattern with authentic standards. Twenty-eight flavonoids were identified and characterized including four flavonoid C-glycosides, twelve flavones, five flavone O-glycosides, three flavonols and four flavonol O-glycosides. This chapter discusses the identification of propenyl phenol derivatives and identification of triterpenic acids and the identification of phenolic acids and their esters. Two isomeric triterpenic acids, peaks 33 and 34, were identified and characterized as oleanolic acid and ursolic acid, respectively. Analysis of phenolic acids and their esters was carried out in negative ionization mode; and eleven phenolic acids and five phenolic acid esters were identified and characterized.