ABSTRACT
The proposed UPLC-multiple reactions monitoring method for quantitative analysis was validated according to the guidelines of International Conference on Harmonization by determining linearity, lower LOD, lower LOQ, precision, solution stability and recovery. The newly developed UPLC-ESI-MS/MS method was applied to quantify 13 bioactive ingredients in the fruit and leaf extracts of ten Piper species, namely, P. nigrum, P. longum, P. chaba, P. umbellatum, P. mullesa, P. colubrinum, P. hymenophyllum, P. argyrophyllum, P. attenuatum and P. galeatum. The clustering of group A was between species leaves of P. umbellatum and fruits of P. colubrinum. Fruits and leaves of Piper species were collected from the plants grown in the herbal garden of Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram, India and Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore, India. The washed and dried fruits and leaves of the Piper species were ground into powder separately.
