ABSTRACT
The use of adenosine triphosphate (ATP) to monitor bacterial growth potential of seawater is currently not possible, as ATP cannot be accurately measured at low concentration in seawater using commercially available luciferase-based ATP detection. The limitation is due to interference of salt with the luciferin-luciferase reaction, which inhibits light production. This research demonstrates that new reagents developed for (i) ATP extraction from microbial cells and (ii) ATP detection in seawater are able to reliably detect microbial ATP as low as 0.3 ng-ATP/L in seawater. The luminescence signal of the new detection reagent is significantly higher (> 20 times) than the luminescence signal of the freshwater reagent, when applied in seawater. ATP can now be used to monitor bacterial growth potential through pre-treatment trains of seawater reverse osmosis (SWRO) plants as the level of detection is significantly lower than required to prevent biological fouling in reverse osmosis membrane systems.
The new reagents have been used to monitor microbial ATP in coastal North Sea water. Moreover, microbial ATP has been applied to monitor the bacterial growth potential (using indigenous bacteria) through the pre-treatment train of an SWRO desalination plant. A significant reduction (> 55 %) of the bacterial growth potential was found through the dual media filtration with 4.5 mg-Fe3+/L coagulant. Overall, the new reagents can detect low microbial ATP concentrations in seawater and can be used to monitor bacterial growth potential in seawater desalination plants.
