ABSTRACT
Suggestions on chemicals have been circulated that are appropriate and suitable for the assessment and evaluation of the sensitivity and specificity of new and modified mammalian cell genotoxicity tests to circumvent misleading positive results. It is appropriate to update these lists of chemicals in view of new available data. The recommended chemicals were revised to fit the following different sets of characteristics:
Group 1 includes chemicals that are in-vivo genotoxins at more than one endpoints, either due to DNA-reactive or non-DNA-reactive mechanisms. Several are known carcinogenics and have a mutagenic mode of action. In this regard, a sub-class of probable aneugens has been introduced.
Group 2 includes chemicals that are usually negative in vivo and follow non-DNA-reactive mechanism. They are either non-carcinogenic or rodent carcinogens with a non-mutagenic mode of action.
Group 3 includes chemicals that are generally negative both in vivo and the Ames test. They are both non-carcinogenic, rodent carcinogenic amid an accepted non-mutagenic mode of action. This chapter updates these recommended lists of chemicals and briefly describes how these should be used for any testing and evaluation.
