ABSTRACT
Messenger RNAs are central components in the expression of eukaryotic structural genes. Thus an important objective for many studies on gene regulation is to isolate specific mRNAs for structure-function analysis, and to prepare specific radioactively labeled cDNA to be used as a hybridization probe for the quantitation of mRNA. Poly(A) mRNA dissociated from polysomes by treatment with 10 mM EDTA and sedimented heterogeneously in dodecyl sulfate-containing sucrose density gradients with a mean sedimentation coefficient of 20S. The sedimentation properties of pulse-labeled and long-term labeled mRNA from highly purified HeLa cell free polysomes, selected for poly(A) content by two successive passages through oligo(dT)-cellulose columns, were analyzed under native and denatured conditions. A comparison of meiotic and postmeiotic mRNAs was made by two demensional gel analyses of their in vitro synthesized translocation products. Among spots identified in the fluorograms of two-dimensional gels, a number of qualitatively new proteins appeared after meiosis.
