ABSTRACT
The subject of spectrophotometry is to study interactions between electromagnetic radiation and matter. If an electromagnetic radiation acts upon a piece of matter and there is selective absorption, the material will absorb different amounts of the components of the radiation of different wavelengths. The change in the degree of absorption as a function of wavelength is the absorption spectrum. According to the wavelength used for the measurement, absorption spectrophotometry can be ultraviolet (UV), visible (VIS), and infrared (IR) spectrophotometry. IR spectrophotometry also enables quantitative determinations on the basis of Beer’s law. On the basis of the position and intensity of a few characteristic bands or knowing the whole spectrum, UV and VIS spectrophotometry can also be used for qualitative analysis.
