ABSTRACT
Biosensors are dedicated to selectively determine different components in complex matrices. Biosensors that employ a biocatalytic process for recognition and an electroanalytical base-sensing element for signal transduction are often called enzyme electrodes. Enzyme electrodes can be considered as the most established, widely studied, and employed electroanalytical biosensors. This chapter describes three different laboratory experiments in which the students prepare different kinds of biocatalytic electrodes and study them in batch-type experiments. Glucose determinations are among the most often performed analytical measurements. Glucose enzyme electrode is made by immobilizing glucose oxidase in a thin film reaction layer on the surface of a platinum disc electrode. A potentiometric pH electrode is used as base-sensing element.
