ABSTRACT
The electro-ejaculation technique of extruding spermatophore from males simplified artificial insemination technique in crustaceans to a certain extent. In an effort to maximize the number of half-sib families and reduce the time that families are produced, a different artificial insemination technique was used to produce paternal half-sib families. In their study12 month-old female shrimp was manipulated to mature using eyestalk ablation and artificial insemination techniques. On the contrary, by adopting artificial insemination technique, selective breeding could be achieved. Artificial insemination technique using refrigerated/ cryopreserved spermotophores in penaeid shrimps and other crustaceans has shown high fertilization and hatch rates which indicates the possibility of commercial applications of this technique for enhancing larval production similar to animal husbandry. Crustacean genetic engineering techniques are of a recent origin with most of the work being carried out on the applied Aquaculture aspects, including brood stock development, heritability, and hybridization, disease diagnostics and also disease therapeutics.
