ABSTRACT
Sophisticated scaffolds are widely used to function as artificial extracellular matrices, and various attempts have been made to produce scaffolds, which have specific structures to regulate cell spreading, package layers of cultured cell sheets, deposit cells directly, or translocate cells. The self-organization are divided into two types: two-dimensional (2D) and three-dimensional (3D) self-organization of cells. 3D cell culture model serves as a suitable fundamental platform for studying the cellular self-organization that mimics the natural development of tissues or trauma repairs. This chapter demonstrates that the self-organized multicellular 3D structure and accommodation of the hydrogel components can be achieved in 3D culture regime.
