DNA barcoding method is based on the fact that the highly conserved regions of DNA, vary minutely within the species during the process of evolution. In DNA barcoding, the complete data can be obtained from single specimen irrespective of sexual morph or life stage. The use of DNA sequences to identify organisms has been proposed as a more efficient approach than traditional taxonomic practices. The sequences suggested to be useful in molecular barcoding include plastid genome, nuclear genome and mitochondrial genome. The Consortium for the Barcode of Life is a global initiative dedicated for developing DNA barcoding as an international standard for the identification of biological species. Barcode of Life Data System provides an integrated bioinformatics platform that supports all phases of the analytical pathway from specimen collection to tightly validated barcode library. Microfluidic Enrichment Barcoding is a cost-effective method of high throughput DNA barcoding proposed by Gostel et al. as an alternative to the traditional method of barcoding.