ABSTRACT
The total carbohydrate content in the isolated biosurfactant (BS) samples was quantified by standard phenol-sulfuric acid method using D-glucose as standard. The lipid content in the isolated BS samples was estimated gravimetrically using a standard protocol of Folch et al. The taxonomic identification of the selected bacterial isolates was carried out using the standard morphological, physiological, and biochemical tests. The bacterial isolates were taxonomically identified up to genus level with the help of standard procedures described in Bergey’s Manual of Systematic Bacteriology. Hemolytic activity was determined on nutrient blood agar media plates containing mammalian blood. Purification of the isolated crude BSs was carried out using thin-layer chromatography with slight modifications. The thermogram of the BS samples revealed two different endothermic peaks. The infrared spectroscopic analysis revealed the presence of glycolipid-type compounds in the isolated BS samples. The Differential scanning calorimetry study was performed on the BSs produced by the bacterial strains.
