ABSTRACT
Chromatographic qualitative and quantitative analysis is based on and justified by the theoretical grounds coming from the theory of plates. These theoretical results justify the experimental values of some parameters such as the chromatographic grandeurs and factors of identification of solutes with the different retention grandeurs, the selectivity factor, the capacity of separation and the qualitative aspects based on several methods such as the overloading and the use of indices type Kovats. The chapter also mentions the fact that quantitative measurements are possible because the chromatographic columns are systematically coupled with differential detectors which give a surface of elution peak proportional to the total quantity of the solute. Methods of calibration to infer the concentrations of solutes from the chromatograms are described and justified. All the notions concerning the resolution of the peaks are also described and justified.
