ABSTRACT
Edman degradation is the preferred technique for determining the primary structure of a protein or peptide. 1 During this process, the amino-terminal residue of the protein is sequentially removed and converted to the phenylthiohydantoin (PTH) amino acid. The PTH derivative can then be identified by thin layer chromatography (TLC), gas chromatography (GC), or high pressure liquid chromatography (HPLC) on re versed-phase supports. HPLC is probably the most commonly used method.
