Gas Chromatography Tables

Wherever possible tables are arranged according to classes of chemical compounds. This was not always possible when different types of compounds were chromatographed under the same experimental conditions. The reader is referred to the compound index for specific compounds which may appear in different tables. Table GC 1 <target id="page_4" target-type="page">4</target>The Influence of the Ester Group on the Separation of <italic>N</italic>-Trifluoroacetyl Amino Acid Esters

Column packing

P1

P1

P1

P1

Temperature

T1

T1

T1

T1

Gas Flow rate (mℓ/min)

He, 30

He, 30

He, 30

He, 30

Column

Length (ft)

6

6

6

6

Diameter (mm, I.D.)

2

2

2

2

Material

G

G

G

G

Detector

FID

FID

FID

FID

t_r(min)

Amino acid

Isopropyl ester

n-Propyl ester

Isobutyl ester

n-Butyl ester

Alanine

0.90

1.11

1.38

1.60

Arginine

5.82

6.18

6.48

6.72

Glutamic acid

4.29

4.87

5.50

5.97

Leucine

1.82

2.22

2.60

2.87

Methionine

3.48

3.92

4.28

4.54

Norleucine

2.13

2.54

2.93

3.20

Phenylalanine

4.08

4.67

5.00

5.25

Proline

2.52

2.98

3.40

3.66

Serine

1.29

1.56

1.87

2.12

Tryptophan-1

8.45

9.20

9.81

10.44

Tryptophan-2

7.09

7.55

7.90

8.31

Note: The incomplete diacylation of tryptophan resulted in two derivatives. Column packing: P1 = 2% OV-17/1% OV-210 on Supelcoport®, 100–120 mesh Temperature: T1 = the temperature was raised from 125–135°C at 10°C/min, thereafter at 20°C/min to 220°C where it was held constant for 10 min Table GC 2 The Influence of the Ester Group on the Separation of <italic>N</italic>-Heptafluorobutyryl Amino Acid Esters

Column packing

P1

P1

P1

P1

Temperature

T1

T1

T1

T1

Gas Flow rate (mℓ/min)

He, 30

He, 30

He, 30

He, 30

Column

Length (ft)

6

6

6

6

Diameter (mm, I.D.)

2

2

2

2

Material

G

G

G

G

Detector

FID

FID

FID

FID

t_r(min)

Amino acid

Isopropyl ester

n-Propyl ester

Isobutyl ester

n-Butyl ester

Alanine

1.19

1.52

1.94

2.30

Arginine

9.31

9.50

9.74

Glutamic acid

6.50

7.51

8.27

8.86

Leucine

2.64

3.32

3.98

4.51

Methionine

5.56

6.25

6.70

7.08

Norleucine

3.20

3.94

4.61

5.11

Phenylalanine

6.74

7.25

7.63

7.98

Proline

3.79

4.64

5.24

5.68

Serine

2.35

2.91

3.44

3.97

Tryptophan-1

11.16

11.77

12.22

12.73

Tryptophan-2

10.05

10.44

10.70

11.06

Note: The incomplete diacylation of tryptophan resulted in two derivatives. Column packing: P1 = 2% OV-1/1% OV-210 on Supelcoport®, 100–120 mesh Temperature: T1 = 120°C for 0.5 min, then raised to 135°C at 5°C/min, then to 220°C at 15°C/min, the final hold time being 13 min