ABSTRACT

The resolution of amino acid enantiomers is important in peptide synthesis and structure determinations. Chromatographic methods of analysis have the advantage of resolving not only a pair of enantiomers, but also a mixture of several amino acid enantiomers. The gas chromatographic separation of D and L amino acids using chiral stationary phases requires the derivatization of amino acids to volatile compounds. For this reason high performance liquid chromatography can be a more attractive procedure.