ABSTRACT
The extracted polymer from the isolated bacteria was dissolved in chloroform in a specific amount and cast over a cover slip homogeneously. The film was kept for 24–36 h at room temperature undisturbed to get a thin film of the potential bacterial polyhydroxyalkanoate (PHA) polymer. The surface topology of the PHA films, incubated in sterile fluid for 36 days and those inoculated with bacteria and fungi were analyzed through optical microscope to verify for any structural change. Images obtained by the SEM analysis on the treated and untreated samples were analyzed on a comparative basis. FTIR analysis was done to detect the degradation of PHA. Genomic DNA from bacteria was prepared as described by Sambrrok et al. Genomic DNA was isolated from bacterial strains P. aeruginosa strains JQ796859 and JQ866912 by using Genei Pure TM Bacterial DNA purification kit.
