ABSTRACT
The potential bacterial polyhydroxyalkanoate (PHA) suspension in distilled water was prepared by sonication for 3 h, using ultrasonic water bath homogenizer. The PHA inclusion bodies in the bacterial species B. circulans. Three different solutions of SNP were prepared in triplicate, each containing 0.08% PHA in 30 mL distilled water. The bright yellow color of the solution is a proof of the presence of SNP with a maximum absorption at about 400 nm, and the solution was labeled as SNPPHA in situ. The colloid of PHA-dispersed solution was spread on TEM (transmission electron microscope). The stub and sample preparations were done using the standard protocol. The growth of P. aeruginosa strain OBP1 on various nanocomposite films such as hyperbranched epoxy (HBE), modified hyperbranched epoxy (MHBE) and their clay nanocomposites along with the corresponding pristine polymeric films can be realized. The FT-IR spectrum of SNP confirmed the presence of silver in the composite.
