ABSTRACT
The lipophilic dyes like Sudan black B, Nile blue A and Nile red are used to dye and detect the hydrophobic potential bacterial polyhydroxyalkanoate (PHA). The lipophilic dye Sudan black B is more soluble in lipid material and imparts a black blue color to the PHA granule. The recovery process of PHA is costly; a variety of methods have been adopted for PHAs extraction from bacterial cells. For the extraction, the bacterial cell biomass is harvested from the culture broth using centrifugation, filtration, and flocculation-centrifugation. The solvent extraction methods for PHAs recovery involve the use of chlorinated solvents like methylene chloride, chloroform, propylene carbonate, or dichloroethane. Sodium hypochlorite digestion method is another recovery method for PHA extraction. Quantification of PHA is done by gravimetric measurement from lyophilized cell biomass. PHAs have been drawing considerable attention as biocompatible plastics for a wide range of applications due to its biocompatibility, biodegradability, and uniform chirality.
