ABSTRACT
Bacterial identification with phenotypic characters is so accurate as compared to genotypic methods. To estimate the relationship between the phylogeny and genotype determination of bacteria, the ribotyping is used as the most common molecular biological approach by comparing the similarities in the rRNA gene sequences. Ribotyping with DNA restriction fragments containing the genes coding for the 16S and 23S rRNA is reliable. 16S-rRNA genes are mainly used for classification and identification of bacteria. For 16S-rRNA gene sequence identification of organisms the concerned databases, appropriate names associated and an accurate sequence of the isolate can be identified. Azotobacter eutrophus bacteria with the diameter of 02–0.5 m contains 8–13 potential bacterial polyhydroxyalkanoate (PHA) granules. The granules appear to be highly refractive inclusion under electron microscopic observation. PHAs are produced by the different bacterial cultures. R. eutropha is mostly used bacteria for the production of PHB and PHBV.
