ABSTRACT
The production of potential bacterial polyhydroxyalkanoate (PHA) from bacteria can be augmented with the culture of concerned bacteria species is the need of the hour. PHA-producing bacteria are classified in to two groups based on culture conditions required for the efficient PHA synthesis. Single fed-batch fermentation that is nitrogen limited lead to low amounts of PHA, as there is not enough accumulation of biomass. The use of activated sludge can contribute to decrease the cost of PHAs and therefore increase their commercial potential. A stable methane-utilizing mixed bacterial culture used in the large-scale production of PHB using cheap substrates like methane from natural or renewable sources in an open system. Several methods have been suggested for the economic recovery of purified PHA. The bacterial cells with PHAs following fermentation are separated by centrifugation, washed, dried, and disrupted to recover the polymer.
