ABSTRACT
Non-condensing polymers forming nanosized vectors with either a neutral or net negative charge have been proposed for tissue and cell specific delivery that possess efficient transfection efficiency with improved cell viabilities. The versatility of PLGA allows chemical modification; several derivatives of PLGA with tailored properties have been designed to fit the delivery purposes. Sustained release of the encapsulated nucleic acids could be achieved by manipulating the degradation rate of PLGA. In one of initial studies, attempts were made to produce cationic PLGA particles using chitosan as a cationic surface modifier for the adsorption of siRNA. Rapid progress has been done in the development of non-condensing delivery systems during the last decade. More and more polymers have been investigated as non-condensing systems for siRNA delivery. The research on non-condensing gene therapy will advance with increased knowledge and innovative delivery strategies.
