ABSTRACT
Poly-L-lysine (PLL) is one of the first polymers investigated for non-viral gene delivery, followed by utilization of a large variety of polymers with different MW in physicochemical and biological experiments. PLL polyplexes are taken up efficiently by the cells as PEI complexes; however, transfection levels remain several orders of magnitude lower. Although PLL has been around for quite a while, it found limited applications in siRNA delivery. PLL presented in different forms e.g. dendrimer, copolymer etc, has been employed to deliver siRNA. The manipulations involving fabrication of targeting moieties or co-application of endosomolytic agents like chloroquine or fusogenic peptides will enhance siRNA efficacies. Proper studies to decipher the mechanism of siRNA delivery employing PLL needs to be done along with studies in vivo animal models to establish the potential therapeutic relevance.
