ABSTRACT
A number of negative staining applications will be given below, taken from my own studies and from the published works of others. These represent a personal selection and no attempt has been made to present a comprehensive survey of the extremely widespread biological and biomedical applications of negative staining. Those requiring further detailed information can readily access the extensive literature via a computer topic or author search. It will be found that negative staining continues to be widely used, alone and increasingly as a preliminary or in parallel with cryoelectron microscopical studies of unstained frozen-hydrated specimens (Norcum et al., 1994).
